UV-light cross-linker-Huatai Hehe (Beijing) Trading Co., Ltd

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UV cross-linking

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The five basic components of ultraviolet visible spectrophotometer are introduced2022-12-7

Detailed introduction

The ultraviolet cross linker is a versatile 245 nm ultraviolet radiation system mainly used for cross-linking and fixing nucleic acids onto membranes. Using this instrument, nucleic acids can be fixed on the hybrid membrane within 30-60 seconds, and the hybridization signal is 5-10 times higher than traditional baking methods. In addition,the instrument can also be used for DNA cutting in agarose gel, RecA mutation screening, partial restriction enzyme digestion produced by pyrimidine dimer, UV sterilization to eliminate PCR pollution, etc.

Technical parameter：

UV wavelength: 254 nm(additional 365nm,312nm tube according to user requirements)

Irradiation time measurement range: 0-999.9min, power 60W

Radiation energy: up to 5mw/cm

UV light source: 5 10W tubes; 9 exposure energies and 9 exposure times can be set and saved; UV exposure energy and UV exposure time are set manually

Exposure energy measurement range: 0-99.9J (Joule)

Exposure time measurement range: 0-999.9min

External dimensions: 360mm*340mm*310mm

Internal exposure chamber size: 340mm*260mm*150mm

TYPE	NM	TUBES
BLX-254	254	5×8W
BLX-312	312	5×8W
BLX-365	365	5×8W

• High efficiency allows nucleic acids to be fixed to the hybrid membrane within 30-60 seconds

• High sensitivity hybridization signal increases 5-10 times compared to traditional baking methods

• Dose precision microprocessor controls precise UV dose, LED display

• Convenient to operate, drawer type design, automatic storage of information

• Large exposure area Maximum exposure area 88cm2

• Mode options include UV energy mode and time mode

• High safety UV safety window and safety door lock design can protect the safety of use

Operating principle：

A UV energy program (Joules/cm2) is configured in the UV detector, in which the time integrator continuously monitors the exposure of UV light. When the energy absorption value of the ultraviolet radiation detector reaches the set value, the irradiation of the ultraviolet radiation detector will automatically stop.

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UV-light cross-linker

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