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Automated microplate reader is an experimental technique widely used in biomedical research and clinical diagnosis. It is based on the principle of enzyme-labeled antigen-antibody reaction, which quantifies the presence and concentration of target substances by measuring the optical signal of the enzyme marker.
Automated microplate readers offer a variety of features and benefits. First of all, it can be used to detect and quantify various substances such as proteins, nucleic acids, hormones, cytokines, etc. This makes microplate readers very widely used in biomedical fields, including the detection of cancer markers, the diagnosis of infectious diseases, and the development of new drugs.
Secondly, the microplate reader has high sensitivity and specificity. Since the signal generated by the enzyme marker can be amplified, very low concentrations of the target substance can be detected. And, due to the specific binding of the enzyme marker to the antigen or antibody, the microplate reader is able to accurately distinguish between the target and other interfering substances, providing reliable results.
Thirdly, the microplate reader has strong adaptability and flexibility. It can be applied to the analysis of small sample sizes, saving experimental materials and costs. In addition, microplate readers can perform high-throughput analysis and process multiple samples at the same time, increasing work efficiency.
Finally, the microplate reader is easy to operate and the results are visualized. By adding the enzyme chromogenic substrate to the reaction system, the color change can be observed, so as to intuitively determine the presence or absence of the target substance. At the same time, the microplate reader is equipped with data analysis software, which can automatically calculate the results and generate reports, reducing human error and providing more accurate quantitative analysis.
Automated microplate readers are an efficient, sensitive, specific, and easy-to-use experimental technique. It plays an important role in biomedical research and clinical diagnosis, providing scientists and physicians with an effective tool to facilitate the early diagnosis and treatment of diseases and advance the field of biomedicine. With the continuous development of technology, it is believed that the microplate reader will play a greater role in the future and make greater contributions to the cause of human health.