Full-wavelength microplate readers play an important role in scientific research and clinical practice

    Enzyme-Linked Immunosorbent Assay (ELISA) is an experimental technique widely used in biomedical research and clinical diagnosis. It uses enzymes as signal amplifiers to detect and analyze target substances through the catalytic action of enzymes, which has the characteristics of high efficiency and accuracy.

    The principle of the microplate reader is based on the immune response. First, an antigen or antibody is immobilized on a microplate to form a specific immune complex. The sample to be tested is then added to the microwells so that the target in the sample binds specifically to the immobilized antigen or antibody. Next, a secondary antibody or substrate-enzyme conjugation reagent is added to form a new complex. Finally, by adding substrates, the signals such as chromogenic reactions or fluorescence catalyzed by enzymes were observed, and the signals were quantitatively measured by microplate readers.

　The advantage of a full-wavelength microplate reader is its high sensitivity and reliability. Since the enzyme acts as a signal amplifier, the signal of the detected substance can be amplified, thus increasing the sensitivity of the detection. In addition, the microplate reader also has a wide range of applications, which can be used to detect proteins, antibodies, cytokines and other biomolecules, and can detect multiple samples at the same time, improving work efficiency.

    In addition, the microplate reader is also simple and easy to operate. Compared with other technologies, such as immunofluorescence and radioimmunoassay, microplate readers do not require complex equipment and means, are easy to operate, and do not involve radiation safety issues, so they are more likely to be widely used in laboratories and clinical practices.

    Microplate readers play an important role in clinical diagnosis. For example, ELISA can be used to detect antibodies, drug concentrations, hormone levels, and more for certain infectious pathogens to help doctors make diagnostic and treatment decisions. In addition, ELISA allows for early screening of many diseases, detecting potential health problems, and promoting early prevention and intervention of diseases.

    As an efficient and accurate bioanalytical tool, microplate spectroscopy plays an important role in scientific research and clinical practice. It not only provides reliable quantitative results, but also enables rapid detection of multiple samples, supporting further research and development in the biomedical field.